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KMID : 0903519910340030273
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1991 Volume.34 No. 3 p.273 ~ p.278
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Production of poly - β - hydroxybutyric acid by methanol assimilating bacterium , Pseudomonas sp . ILS - 003
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Abstract
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Methanol assimilating bacterium, Pseudomonas sp. ILS-003 was used to investigate the optimum conditions for the production of poly-β-hydroxybutyric acid from methanol. For PHB production, the optimum initial pH was 6.4 and the optimum temperature was 30℃. Also the optimum methanol concentration was found to be 1.0%(v/v). In the PHB production, (NH₄)₂SO₄ was the most effective nitrogen source and the optimum concentration was 0.8 g/ℓ, which was eqivalent to 17.4 in C/N ratio. Also, deficiency of the 2 valence metal ions in the medium had stimulating effect on PHB accumulation. Under the optimum substrate concentration, successive feeding of 0.25%(v/v) methanol was the most effective on PHB production. Under the optimum conditions, 1.94 g/ℓ of PHB and 2.78 g/ℓ of dry biomass were produced in 96 hours, and the yield was 69.8%(w/w).
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